![Flow Cytometry: Human TLR3 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26275] - Intracellular staining of TLR3 in the TLR3 reporter cell line was analyzed by flow cytometry using a PE-conjugated anti-TLR3 antibody. Flow samples were prepared using the Intracellular TLR Staining Flow Kit. Purple: Cells alone; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 1ug anti-TLR3-PE; Red: TLR3 reporter cell line stained with 1 ug anti-TLR3-PE.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10743_Human-TLR3-NF-kB-SEAP---(SEAPorter(TM))-Stable-Reporter-Cell-Line-Flow-Cytometry-NBP2-26275-img0005.jpg)
Flow Cytometry: Human TLR3 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26275] - Intracellular staining of TLR3 in the TLR3 reporter cell line was analyzed by flow cytometry using a PE-conjugated anti-TLR3 antibody. Flow samples were prepared using the Intracellular TLR Staining Flow Kit. Purple: Cells alone; Green: NF-kB/SEAPorterTM HEK 293 cell line stained with 1ug anti-TLR3-PE; Red: TLR3 reporter cell line stained with 1 ug anti-TLR3-PE.
![Ligand Activation: Human TLR3 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26275] - TLR3/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of poly(I:C) for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. poly(I:C)-mediated TLR3 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of poly(I:C). The EC50, or the half maximal activity concentration, represents the concentration of poly(I:C) that was required for 50% activation of TLR3 as measured by SEAP activity. Data Summary: Poly(I:C) activated thecell line in a dose response manner, of which EC50 was measured as 22.07 ug/ml.](http://www.bioprodhub.com/system/product_images/cel_products/5/sub_2/10744_Human-TLR3-NF-kB-SEAP---(SEAPorter(TM))-Stable-Reporter-Cell-Line-Ligand-Activation-NBP2-26275-img0006.jpg)
Ligand Activation: Human TLR3 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26275] - TLR3/NF-kB SEAPorterTM HEK 293 cells were plated in 96-well plates at 5 x 10^4 cells/well. After 16 h, cells were stimulated with various amount of poly(I:C) for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. A. poly(I:C)-mediated TLR3 activation, as measured by SEAP activity, was increased in a dose dependent manner. B. The values from (A) were used to determine the EC50 of poly(I:C). The EC50, or the half maximal activity concentration, represents the concentration of poly(I:C) that was required for 50% activation of TLR3 as measured by SEAP activity. Data Summary: Poly(I:C) activated thecell line in a dose response manner, of which EC50 was measured as 22.07 ug/ml.